FIGURE 2.

GW9508 inhibits RANKL-induced osteoclast differentiation in primary cell cultures obtained from mouse bone marrow tissues. A and B, TRACP staining: wild-type (WT) versus GPR40^−/− mice (KO). Magnification, ×10 (Zeiss). Osteoclast differentiation was induced by murine recombinant RANKL (50 ng/ml). DMSO was used as a vehicle. GPR40 agonist GW9508 was used at either 10 or 50 μm (GW10 or GW50). C, osteoclast marker expression analyzed by real-time RT-PCR. Error bars represent standard deviation (±S.D.); *, p < 0.05.