FIGURE 4.

GW9508 inhibits RANKL-induced signaling pathways in RAW264.7 cell cultures. A, Western blot analysis of IkBα and IKKα/β phosphorylation. B, NF-κB-dependent luciferase assay. DMSO was used as a vehicle. GPR40 agonist GW9508 was used at 50 μm (GW50). Relative light units were measured at 6 and 24 h after incubation and reported to total protein content. C, NFATc1 expression analyzed by real-time RT-PCR in cells invalidated for GPR40 expression (Sh) or transfected with a nontargeting vector (SCR). *, p < 0.05. DMSO was used as a vehicle. GPR40 agonist GW9508 was used at either 10 or 50 μm (GW10 or GW50). Error bars represent standard deviation (±S.D.).