GPR40 agonist as a protective agent against in vivo OVX-induced bone loss.
A, bone microarchitecture (females; OVX, ovariectomy; Sh, Sham-operated; 14-week-old mice; n = 7 per group; BV/TV, bone volume/total volume; Tb.N, trabecular number; Tb.Sp, trabecular spaces; Tb.Th, trabecular thickness). Mice received either DMSO as vehicle or GPR40 agonist GW9508 (8 mg/kg of body weight: GW). B, representative bone tissue marker expression analyzed by TLDA on whole tibiae (Applied Biosystems 7900HT real-time PCR system). Tissues were collected from OVX or Sham-operated mice that received either DMSO as vehicle or GPR40 agonist GW9508 (8 mg/kg of body weight: GW). Different letters are attributed to significantly different groups (p < 0.05); error bars represent standard deviation (±S.D.).