FIGURE 3.

Gadd45a knockdown HeLa conditioned media promote tube formation and migration of endothelial cells. The media conditioned by Gadd45a knockdown HeLa (si1 and si2) were collected. Tube formation (a) and migration assays (b) of HUVECs were performed following the procedure described in Fig. 2. NC, negative control. c, the number of tube structures/field was presented as mean ± S.E. n = 3. **, p < 0.01. d, the number of cells that migrated through the membrane/field was presented as mean ± S.E. n = 3. **, p < 0.01. Scale bar = 500 μm (a) and 100 μm (b). e, Western blot analysis evaluated the efficiency of Gadd45a knockdown.