FIGURE 12.

Slow blocker binding in MTSEA-modified 169C and 212C channels. A, exemplar data illustrating the effects of MTSEA modification of Kir6.2[S212C] dimeric channels on blocking kinetics of PG-11098 or spermine (both at 100 μm). Patches were pulsed from −80 mV to +50 mV after the indicated manipulations. Pre-mod, before modification. B, summarized data describing spermine and PG-11098 blocking kinetics in unmodified and MTSEA-modified 169C and 212C channels (n = 3–5 per recording condition). MTSEA modification of these positions profoundly slows blocker binding. Error bars indicate S.E.