FIGURE 2.
Comparison of inhibition of Na,K-ATPase activity by glycosylated and unglycosylated CTSs at pH 7.5. A, inhibition by digitoxin (Ki = 167 nm), digitoxigenin (Ki = 176 nm), and evomonoside (Ki = 105 nm). B, inhibition by digoxin (Ki = 147 nm) and digoxigenin (Ki = 194 nm). C, inhibition by gitoxin (Ki = 147 nm) and gitoxigenin (Ki = 403 nm). D, inhibition by ouabain (Ki = 89 nm) and ouabagenin (Ki = 2282 nm). Mean Ki values calculated from fitting to the sigmoid dose-response equation are given in Table 1.