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. 2013 Jan 18;288(9):6640–6650. doi: 10.1074/jbc.M112.432682

FIGURE 6.

FIGURE 6.

14-3-3-uncoupled Syx is strongly targeted to the cell border and enhances circumferential actin accumulation. A, Syx S92A/S938A localizes strongly at areas of cell-cell contact. MDCK cells were transfected with plasmids encoding YFP-Syx or YFP-Syx S92A/S938A. Cells were fixed and immunostained for YFP, ZO1, and DAPI. B, Syx S92A/S938A induces the accumulation of circumferential actin at cell contacts. MDCK cells were transfected with the indicated constructs, fixed 24 h after transfection, and immunostained for YFP, phalloidin (F-actin), and DAPI. C, A quantitative analysis of enhanced circumferential actin staining in transfected cells from B was performed. The intensity of circumferential actin at the junctions of transfected cells was compared with that of immediate neighboring nontransfected cells using ImageJ; transfected cells that exhibited intensified circumferential actin staining are expressed as percentage of total cells counted (mean ± S.E. (error bars), n = 3, 50 cells/coverslip analyzed). Scale bars, 10 μm.