FIGURE 8.
TNF does not alter global levels of histone acetylation or histone H3K9 acetylation but does increase histone H4K8 acetylation in the −837 to −618 Gαs promoter fragment. Primary cultures of myometrial cells were stimulated with TNF (10 ng/ml) for 24 h and subjected to the ChIP assay with the antisera depicted. Data were compared using a paired, two-tailed t test; p < 0.05 was considered statistically significant. All experiments were performed three times, and results are expressed as the mean ± S.E. (error bars). TNF did not alter the global levels of acetylated lysine-containing proteins (A) or acetylated histone H3K9 (B) within the endogenous Gαs promoter, but it induced an ∼3-fold increase in acetylated histone H4K8 levels in the endogenous −837 to −618 Gαs promoter region (C, panels i–iii). I, input; IP, immunoprecipitate.