Table 4. Alkaloid profiles of sequenced isolates.a .
Strain | ||||||||||||||
Alkaloid | C f u | C p a | C p u | E a m | E b e | E e l | E f1 | E f2 | E g l | E t8 | N g a | N g i | N u n | P i p |
Ergot alkaloids: | ||||||||||||||
Chanoclavine (CC) | + | (+) | nt | + | (+) | (−) | (−) | + | + | |||||
Agroclavine | + | (+) | − | (+) | (−) | (−) | (+) | + | ||||||
Elymoclavine (EC) | + | (+) | − | (+) | (−) | (−) | (+) | (+) | ||||||
Lysergic acid | − | (+) | − | (+) | (−) | (−) | (+) | (+) | ||||||
Lysergic acid amide | − | nt | − | − | − | − | + | + | ||||||
Ergonovine (EN) | − | nt | − | − | − | − | + | + | ||||||
Lysergic acid α-hydroxyethylamide (LAH) | − | − | − | − | − | + | + | |||||||
Ergopeptine | − | ERA ERC | − | ERV | (−) | (−) | − | ERB | ||||||
Indole-diterpenes: b | ||||||||||||||
Paspaline | nt | + | + | + | ||||||||||
Paspaline B | nt | + | + | + | ||||||||||
13-Desoxypaxilline | + | + | + | |||||||||||
Paxilline (PAX) | − | + | + | |||||||||||
IDT-436 | − | − | + | |||||||||||
Terpendole E | (+) | − | + | |||||||||||
Terpendole I | (+) | − | + | |||||||||||
Terpendole J | (+) | − | + | |||||||||||
Terpendole C | + | − | + | |||||||||||
Terpendole K (TDK) | − | − | + | |||||||||||
Terpendole M | − | − | + | |||||||||||
Terpendole N | − | − | + | |||||||||||
Terpendole A | − | − | + | |||||||||||
Lolicine A | + | − | − | |||||||||||
Loliline | + | − | − | |||||||||||
Lolitrem E | + | − | − | |||||||||||
Lolitrem B (LTM) | + | − | − | |||||||||||
Lolines: | ||||||||||||||
Acetamidopyrrolizidine (AcAP) | (+) | + | (+) | (+) | + | |||||||||
N-Acetylnorloline (NANL) | + | − | + | + | + | |||||||||
Loline | − | − | + | − | + | |||||||||
N-Acetylloline | − | − | + | − | + | |||||||||
N-Methylloline | − | − | + | − | + | |||||||||
N-Formylloline (NFL) | − | − | + | − | + | |||||||||
Peramine (PER) | + | nt | + | + | − | + |
Strains are abbreviated as follow: Cpu = Claviceps purpurea 20.1, Cfu = C. fusiformis PRL 1980, Cpa = C. paspali RRC-1481, Eam = Epichloë amarillans E57, Ebe = E. brachyelytri E4804, Eel = E. elymi E56, Ef1 = E. festucae Fl1, Ef2 = E. festucae E2368, Egl = E. glyceriae E2772, Et8 = E. typhina E8, Et5 = E. typhina E5819, Nga = N. gansuense E7080, Ngi = N. gansuense var. inebrians E818, Nun = N. uncinatum E167, Pip = P. ipomoeae IasaF13. Symbols: + = present, (+) = intermediate inferred to be synthesized because downstream product is present, − = not predicted and not detected, (−) = predicted but not detected, nt = predicted but not tested, ERA = ergotamine, ERB = ergobalansine, ERC = ergocryptine, ERV = ergovaline. Blank cells indicate compounds not predicted from genotype, and not tested.
Identification of IDT-436 and terpendoles E, I, J, K, M, M, and A are tentative because authentic standards are unavailable.