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. 2013 Feb 28;9(2):e1003277. doi: 10.1371/journal.pgen.1003277

Figure 3. ATM-mediated phosphorylation of CtIP is important for end resection in a CDK-dependent manner.

Figure 3

A. EGFP-HR assay was performed with U2OS cells stably expressing HA-CtIP WT or indicated mutants, with endogenous CtIP silenced. Data represent the mean ± s.d. of three independent experiments. Western blot shows expression of HA-CtIP variants. B. Clonogenic survival assay after treatment with indicated doses of CPT (1 h) was performed with U2OS cells stably expressing indicated CtIP variants with endogenous CtIP silenced. Data represent the mean ± s.d. of three independent experiments. C. RPA foci formation was examined in U2OS cells with indicated CtIP variants, before or after CPT treatment. The percentage of RPA foci-positive cells was determined as in Figure 1F. D. U2OS cells stably expressing indicated CtIP variants with endogenous CtIP silenced were treated with IR (10 Gy, recovered for 1 h), lysed, and immunoblotting was performed. E. EGFP-HR repair assay, clonogenic survival and RPA foci formation assays were performed in U2OS cells expressing HA-CtIP-WT or indicated mutants with endogenous CtIP silenced. F. EGFP-HR assay was performed with U2OS cells stably expressing HA-CtIP WT or indicated mutants, with endogenous CtIP silenced. Data represent the mean ± s.d. of three independent experiments. Western blot shows expression of HA-CtIP variants.