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. 2013 Feb 28;8(2):e57792. doi: 10.1371/journal.pone.0057792

Figure 3. Affect of mutations in the 17 bp direct repeats on precise excision of the vlsE variable region.

Figure 3

A) DNA sequences of the wild-type 17 bp direct repeat (DR) and a mutant 17 bp direct repeat (DR*) used in this study. Mutated bases are highlighted in red. B) Schematic showing the plasmid templates carrying wild-type DRs and a mutant DR at the left, right or both sides of the variable region. C ) An ethidium bromide-stained agarose gel showing amplification of a portion of vlsE with Phusion DNA polymerase using the templates shown in Panel B with the indicated primers. Gel electrophoresis conditions were as noted in Fig. 2.