Figure 5. The affect of DNA strand and mutations in the G–motif on quadruplex formation.

A,C) Oligonucleotides corresponding to the wild-type 14-mer and 17-mer top (see Fig. 4) and bottom strands, and mutated top strands in which four successive G’s were substituted with TATA. B,D) Autoradiogram of a 20% native gel run to assess G4 formation, as described in Fig. 4.