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. 2013 Feb 28;9(2):e1003169. doi: 10.1371/journal.ppat.1003169

Figure 4. Globodera pallida flp-32 (Gp-flp-32) and FLP-32 receptor (Gp-flp-32R) silenced nematodes exhibit accelerated migration rates.

Figure 4

siRNA soaking reduces Gp-flp-32 and Gp-flp-32R transcript levels by 55% and 75% respectively (A) compared to controls (control siRNA and untreated worms; P<0.001). Post-RNAi Gp-flp-32 and Gp-flp-32R silenced worms displayed an increased speed of migration down a vertical sand column when compared to control worms (B and C). After 4 h migration 84% of Gp-flp-32 and 91% of Gp-flp-32R worms had migrated compared to 59% and 64% of control siRNA and untreated worms respectively (P<0.01; B and C), after 6 h all Gp-flp-32 and Gp-flp-32R silenced worms had completed migration (B and C). There was no significant difference in the migratory ability of Gp-flp-32 and Gp-flp-32R silenced worms over the 6 h migration period (C).