Figure 3. Nuclease activity and substrate specificity of recombinant GST-MAG_5040.

Approximately 4 µg of rGST-MAG_5040 were incubated with calf thymus dsDNA (top left panel), closed circular plasmid DNA (pGEX-2T/rMAG_5040, top right panel), phage M13 ssDNA (bottom left panel), or E. coli total RNA (bottom right panel). An aliquot of each reaction mixture was analysed at different times, as indicated for each lane. C indicates endpoint reaction of the negative undigested control (GST only). Both 1 Kb (upper panels) and 1 Kb Plus (bottom panels) DNA ladders were used (Invitrogen) and indicated as MW in far left lanes of each panel.