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. 2013 Feb 28;8(2):e57823. doi: 10.1371/journal.pone.0057823

Table 1. Gradient elution program for HPLC-ELSD-based separation.

Time (min) 0 4 5 10 12 15 18 20 25 30 40
A(%) 100 100 85 80 75 50 30 30 25 30 100
Solvents B(%) 0 0 15 20 25 50 50 40 30 70 0
C(%) 0 0 0 0 0 0 20 30 45 0 0
Flow rate (ml/min) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0