Table 5. Microsatellite analysis of cloned piglets derived from fetal fibroblasts.
| Marker | Dye name | PCR annealing temp | Genotypes of Recipient | Cell line genotypes (BN133) | Genotypes of litter | |||||||||
| 1 | 2 | 3 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||||
| S0026 | FAM | 55 | 97 | 93/101 | 91/97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 |
| S0070 | FAM | 55 | 271/273 | 285/287 | 263 | 271/273 | 271/273 | 271/273 | 271/273 | 271/273 | 271/273 | 271/273 | 271/273 | 271/273 |
| S0155 | FAM | 55 | 142/152 | 142/152 | 152/156 | 142 | 142 | 142 | 142 | 142 | 142 | 142 | 142 | 142 |
| S0226 | FAM | 55 | 183/195 | 193 | 183 | 195 | 195 | 195 | 195 | 195 | 195 | 195 | 195 | 195 |
| SW122 | FAM | 55 | 108 | 108/110 | 106/110 | 108 | 108 | 108 | 108 | 108 | 108 | 108 | 108 | 108 |
| SW24 | FAM | 55 | 103 | 101/103 | 115 | 103 | 103 | 103 | 103 | 103 | 103 | 103 | 103 | 103 |
| SW72 | FAM | 55 | 97 | 99/109 | 97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 |
| SW830 | FAM | 50 | 180/184 | 168 | 180 | 180/184 | 180/184 | 180/184 | 180/184 | 180/184 | 180/184 | 180/184 | 180/184 | 180/184 |
| SW840 | FAM | 55 | 135 | 117/123 | 125/129 | 135 | 135 | 135 | 135 | 135 | 135 | 135 | 135 | 135 |
| SW857 | FAM | 55 | 139/155 | 139/151 | 143/153 | 139 | 139 | 139 | 139 | 139 | 139 | 139 | 139 | 139 |
| SW936 | FAM | 55 | 97 | 95/109 | 93/119 | 97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 | 97 |
*
For each microsatellite marker, genotype was determined by size (base pairs). Two numbers for each sample at each locus represent the PCR product size at that particular locus.
*
Litters 1,2,3 came from recipient 1; litters 4,5,6 came from recipient 2; and the rest came from recipient 3.