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. 2013 Feb 28;8(2):e57579. doi: 10.1371/journal.pone.0057579

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© 2013 Takacs et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A). Overlay of HPLC profiles of muropeptides obtained from C. crescentus cultures grown in PYE (black trace) and M2G (red trace) media. Black arrowheads, Penta(Gly)-containing muropeptide peaks present only in the PYE-derived sample; red arrowhead, peak corresponding to the PentaTri muropeptide species, identified in the M2G-derived sample. (B). Relative representation (molar percentage) of each muropeptide species in PG digests obtained from C. crescentus cultures grown in PYE (black) and M2G (grey) media. Red rectangles denote glycine-containing species. Tri, GlcNAc-MurNAc-l-Ala-d-γ-Glu-m-Dap; Tetra, GlcNAc-MurNAc-l-Ala-d-γ-Glu-m-Dap-d-Ala; Penta, GlcNAc-MurNAc-l-Ala-d-γ-Glu-m-Dap-d-Ala-d-Ala; Penta(Gly), GlcNAc-MurNAc-l-Ala-d-γ-Glu-m-Dap-d-Ala-Gly; Anh, 1,6-anhydro- MurNAc; (D,D), m-Dap-D-Ala crosslink; (L,D), m-Dap-m-Dap crosslink. Bars represent averages ± standard deviation for the three samples analyzed. (C). Summary of the composition of C. crescentus PG digests obtained from cultures grown in the indicated media. Major PG characteristics are shown, namely the total degree of crosslinkage, the relative amounts of differentially crosslinked muropeptide classes (monomers, dimers, trimers and tetramers), side chain types (Tri, l-Ala-d-γ-Glu-m-Dap; Tetra, l-Ala-d-γ-Glu-m-Dap-d-Ala; Penta(Ala), l-Ala-d-γ-Glu-m-Dap-d-Ala-d-Ala; Penta(Gly), l-Ala-d-γ-Glu-m-Dap-d-Ala-Gly), or chain ends (anhydro, 1,6-anhydro-MurNAc ). Bars are as in (B). The red rectangle highlights the relative representation of the Penta(Gly) side chain. (B and C).