Table 3. Changes in the muropeptide composition of C. crescentus PG following amino acid supplementation of the defined growth medium M2G.
Muropeptide | Molar Percentage (Mean ± Standard deviation) | |||
M2G (n = 2) | M2G + 0.2 mM Gly(n = 2) | M2G + 0.1% CAA(n = 3) | M2G + 2 mM Gly(n = 2) | |
Tri | 0.68±0.02 | 0.68±0.15 | 0.97±0.05 | 0.66±0.01 |
Tetra | 24.97±0.07 | 25.28±0.28 | 29.68±0.44 | 20.39±0.23 |
Penta(Gly) | 0.03±0.04 | 0.05±0.01 | 1.20±0.06 | 10.50±0.05 |
Penta | 12.49±0.31 | 12.13±0.32 | 10.61±0.13 | 11.29±0.44 |
TetraTri(D,D) | 1.20±0.08 | 1.14±0.33 | 3.31±1.19 | 1.24±0.21 |
Tri-Anh (+ small amount of TetraTri(D,L)) | 0.51±0.00 | 0.50±0.06 | 0.56±0.24 | 0.38±0.25 |
TetraPenta(Gly) | N.D.* | N.D.* | 2.20±0.13 | 8.94±0.58 |
PentaTri(D,L) | 0.29±0.01 | 0.41±0.06 | N.D.* | N.D.* |
TetraTetra | 15.67±0.17 | 16.20±0.79 | 15.42±0.20 | 10.29±0.32 |
TetraPenta | 9.47±0.20 | 9.33±0.06 | 6.36±0.10 | 7.37±0.17 |
Tetra-Anh | 1.16±0.11 | 0.95±0.08 | 1.34±0.07 | 0.97±0.09 |
TetraTetraPenta(Gly) | 0.06±0.02 | 0.05±0.07 | 0.16±0.00 | 1.64±0.24 |
TetraTetraTetra | 3.32±0.04 | 3.42±0.13 | 2.63±0.04 | 1.79±0.06 |
TetraTetraPenta | 1.92±0.04 | 1.88±0.04 | 0.98±0.07 | 1.27±0.04 |
TetraTetraTetraTetra | 0.45±0.03 | 0.28±0.12 | 0.22±0.01 | 0.10±0.14 |
TetraTetraTetraPenta | 0.60±0.01 | 0.60±0.10 | 0.40±0.01 | 0.60±0.14 |
Penta-Anh | 0.28±0.01 | 0.28±0.01 | 0.17±0.01 | 0.19±0.05 |
TetraPenta(Gly)-Anh | 0.08±0.01 | 0.03±0.04 | 0.38±0.06 | 1.67±0.14 |
TetraTetra-Anh | 3.99±0.01 | 4.07±0.16 | 4.29±0.10 | 2.66±0.11 |
TetraPenta-Anh | 2.19±0.18 | 2.21±0.19 | 2.43±0.31 | 4.65±0.27 |
TetraTetraTetra-Anh | 6.71±0.21 | 6.81±0.36 | 6.03±0.12 | 4.13±0.10 |
TetraTetraPenta-Anh | 6.32±0.12 | 6.26±0.20 | 4.49±0.13 | 3.95±0.26 |
TetraTetraTetraPenta(Gly)-Anh | 0.38±0.03 | 0.39±0.05 | 0.31±0.05 | 0.46±0.37 |
TetraTetraTetraPenta-Anh | 1.54±0.31 | 1.54±0.31 | 0.83±0.10 | 0.80±0.30 |
TetraTetraTetra-diAnh | 1.44±0.40 | 1.24±0.72 | 1.53±0.07 | 0.96±0.07 |
TetraTetraTetraPenta-diAnh | 1.72±0.20 | 1.70±0.15 | 1.51±0.05 | 1.08±0.08 |
TetraTetra-diAnh | 1.71±0.10 | 1.83±0.29 | 1.47±0.11 | 1.38±0.37 |
TetraTetraPenta-diAnh | 0.83±0.15 | 0.73±0.11 | 0.50±0.05 | 0.64±0.15 |
N.D. Not detected.