Figure 1. Schematic diagram of pc-Rel-luc reporter construct and identification of the transgenic founders.

(A) The c-Rel-luc transgene harboring a 14.6 kb mouse Rel gene promoter and firefly luciferase cDNA was diagramed. Primer P1 and P2 was indicated. P1: forward-luc primer, P2: reverse-luc primer. (B) A 604-bp fragment was amplified by PCR with primer P1 and P2 in transgenic founder mice. PCR products were separated on 1% agarose gel. Lane 1–9: the products from transgenic founders; lane 10: from a wild-type C57BL/6 control; lane 11: from ddH₂O; lane 12: DNA marker DL 2,000 ladder.