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. 2013 Feb 28;8(2):e57829. doi: 10.1371/journal.pone.0057829

Figure 6. Tbx18 and Wt1 are bound to the Slug promoter region and regulate the activity of the Slug promoter.

Figure 6

(A) Immunoblot performed with an anti-Tbx18 antibody and an anti-Wt1 antibody. Tbx18 was immunoprecipitated with an anti-FLAG antibody (FLAG) or control IgG (IgG) in NMuMG-C7 cells expressing 3×FLAG-tagged Tbx18, and Wt1 was immunoprecipitated with an anti-Wt1 antibody (Wt1) or control IgG (IgG) in NMuMG-C7 cells expressing Wt1. (B) Direct binding of Tbx18 or Wt1 near the transcription start site (TSS) of the Slug gene in NMuMG-C7 cells, as assessed by ChIP. DNA fragments co-precipitated with Tbx18 or Wt1 were quantified by real-time PCR. The data are presented as the mean ± SD; n = 3; *P<0.05 vs. control IgG. (C) The relative luciferase activity of a reporter construct carrying the Slug promoter in primary epicardial cells. The data are provided as ratios to siControl and are presented as the mean ± SD; n = 4; *P<0.01 vs. siControl. (D) The relative luciferase activity of a reporter construct carrying the Slug promoter (Full) or the Slug promoter lacking the region around −200 from the TSS (−200 del) or +350 from the TSS (+350 del) in primary epicardial cells. The data are provided as ratios to Full and are presented as the mean ± SD; n = 4; *P<0.05 vs. Full.