Table 2. The oligonucleotide primers and probes used in this study.
Name | Purpose | Sequence 5′→3′ |
Cytb_F | Forward sequencing primer partial cytochrome b gene | GAGCGACTGTAATTACTAATC |
Cytb_R | Reverse sequencing primer partial cytochrome b gene | GGACCCGGATAATTTTGTTG |
Phum540560_F | Forward sequencing primer partial Phum_PHUM540560 gene | GTCACGTTCGACAAATGTT |
Phum540560_R | Reverse sequencing primer partial Phum_PHUM540560 gene | TTTCTATAACCACGACACGATAAAT |
BL probe | Specific to the body lice | FAM-CGATCACTCGAGTGAATTGCCA-TAMRA |
HL probe | Specific to the head lice | VIC-CTCTTGAATCGACGACCATTCGCT-TAMRA |