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. 2013 Feb 28;8(2):e56854. doi: 10.1371/journal.pone.0056854

Figure 8. Linear Bi-Swap TRAS.

Figure 8

The schematic design of linear Bi-Swap TRAS containing the unit vectors C-DNA-G, G-DNA-K, K-DNA-C, G-DNA-C, K-DNA-G and C-DNA-K (A). The yellow round hairpin ends represent N15 plasmids. The chloramphenicol, gentamycin and kanamycin resistance genes are designated cat, gen and kan, respectively. The assembly process for five DNA fragments DNA1, DNA2, DNA3, DNA4 and DNA5 (B). Because this is a linear plasmid system, theoretically no fusion intermediate could form after recombination. PCR and counter-selection may be necessary to indentify the correct products to avoid the false positive case where two unit plasmids exist in the same cell.