Figure 4. Expression of CHOP and Caspase-12 mRNA in hippocampus were analyzed by real-time RT-PCR.

The real time reactions were performed in duplicates for both the target gene and GAPDH used as a housekeeping control. The relative expression was calculated using 2^−ΔΔCt method. Data are presented as mean ± SD. **P<0.01, vs control group.