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. 2013 Feb 28;8(2):e55174. doi: 10.1371/journal.pone.0055174

Figure 7. Physiological levels of human MIC-1/GDF15 reduce weight and food intake in mice.

Figure 7

Male MIC-1−/− and MIC-1+/+ mice were infused with human MIC-1/GDF15 (1ug/20gBW/d) or vehicle via osmotic mini-pump. Food intake, body weight and serum levels of human MIC-1/GDF15 were measured on day 5 of infusion. (A) MIC-1/GDF15-treated MIC-1−/− mice had an average serum MIC-1/GDF15 level of 643±67 pg/ml and weighed 95.86±0.77% of their starting body weight whilst vehicle-treated mice weighed 102.3±0.75% of their starting weight (n = 6/group, p<0.01 unpaired t-test). (B) MIC-1/GDF15-treated MIC-1+/+ mice had an average serum MIC-1/GDF15 level of 576±45 pg/ml and weighed 99.86±0.47% of their starting weight whilst vehicle-treated mice weighed 102±0.52% (n = 14, p = 0.01 unpaired t-test). This decreased body weight in both genotypes was associated with reduced food intake. (C) MIC-1/GDF15-treated MIC-1−/− and (D) MIC-1/GDF15-treated MIC-1+/+ consumed significantly less food than the matched vehicle-treated mice of same genotype (MIC-1−/− n = 6/group, p = 0.04; MIC-1+/+ n = 14/group, p<0.01 unpaired t-test). Data expressed as mean ± SE.