Table 1. Primers used in this study.

Primer	Directiona	Sequenceb	Target gene product	Product size (bp)
ldh-p01	Fw(ApaI)	5′-agggggccctctcgaacagcagtgatacc-3′	ldh promoter	1,110
ldh-p02	Rev(XhoI)	5′-cgcctcgagacagcaccatcaccaacaag-3′
phluorin-01	Fw(XhoI)	5′-cgcctcgagagtaaaggagaagaacttttca-3′	Ecliptic pHluorin gene	723
phluorin-02	Rev(SmaI-SphI)	5′-tcccccgggggacatgcatgctttgtatagttcatccatgcc-3′
ovpcr-a	Fw(XhoI)	5′-cgcctcgagatgaaagtcaaaaaaacttacgg-3′	Signal peptide and first two amino acids coding gene of spaP	120
ovpcr-b	Rev	5′- gaaaagttcttctcctttactttcatcggcaaaaaccttttg-3′
ovpcr-c	Fw	5′-caaaaggtttttgccgatgaaagtaaaggagaagaacttttc-3′	Ecliptic pHluorin gene and linker	747
ovpcr-d	Rev	5′-tcactagtagtggtcgtaccggtcccgccgcttccgccgctggacatgcatgctttgta-3′
ovpcr-e	Fw	5′-tacaaagcatgcatgtccagcggcggaagcggcgggaccggtacgaccactactagtga-3′	spaP gene absence of signal peptide and first two amino acids coding sequence	4569
ovpcr-f	Rev(NotI-SphI)	5′-ataagaatgcggccgctaaactatacatgcatgctcaatctttcttagcctttaagc-3′

^aFw, forward; Rev, reverse.

^bRestriction site sequences are underlined.