. 2013 Feb 28;8(2):e57182. doi: 10.1371/journal.pone.0057182

Table 2. Strains and plasmids used in this study.

Strain	Relevant characteristics	Reference
E. coli DH5α	supE44 lacU169 (80 lacZ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 luxS	[28]
S. mutans UA140	Wild-type S. mutans Kan^s Erm^s	[29]
S. mutans strain O87	UA140: : Φ(ldh_p-leading sequence-gfp-linker-spaP)	This work
Plasmid
pGM87	Superecliptic pHluorin gene in pGEX-2T; Amp^r	kindly provided by Dr. Gero Miesenbőeck
pBluescript II SK (−)	Cloning vector; Amp^r	[30]
pFW5	shuttle suicide vector; Spec^r	[31]
pFW5-ldh_p-leading sequence -gfp-linker-spaP	Ecliptic pHluorin gene under ldh promoter, inserted between the second and third amino acids after the identified signal-peptide cleavage site of surface protein antigen SpaP, with a linker downstream of gfp; Spec^r	This work