Table 2. RNA amount cut-off values for qRT-PCR analysis using archival cytology smears.

Material	Gene	RNA amount cut-off	Slope	R-squared	Efficiency
Cell lines	RNA U6B	1 ng	−3.561	0.99	1.81
	miR-205	5 ng	−3.649	0.91	1.75
Non-tumor urine smears	RNA U6B	10 ng	−2.966	0.99	2.37
	miR-205	20 ng	−3.408	0.99	1.94