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Examples of PSFs measured using (A) water and (B) oil immersion objectives on the coverslip (i) and on top of a live cardiac myocyte (ii) bathed in 1 mM Ca-Tyrode's solution are shown (see inset). Scale bars indicate 0.5 µm. (C) The intensity profiles across the peak intensity in (i) y and (ii) z are shown. The effect of the refractive index mismatch across the myocyte was more pronounced when using an oil immersion objective.
