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. 2013 Feb 28;9(2):e1002931. doi: 10.1371/journal.pcbi.1002931

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© 2013 Kong et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) shows the simulated line-scan images of [Ca]_SR (purple), Ca-Fluo-5N (green) and Ca blink (blurred Ca-Fluo-5N, red) signals when the Ca sparks were simulated with an cell-top PSF. Left panels in (B) and (C) show the time and spatial profiles through the minimum Ca blink intensity, respectively. The right panels show scaled versions of the profiles.