Table 1. Computer model buffers: concentrations, diffusion coefficients and binding kinetics.
x | [x] | D | Kon | Koff | KD |
(µM) | (10−8 dm2/s) | (/µM/s) | (/s) | (µM) | |
Cytosol | |||||
Cafree | 0.1 | 3.0 | - | - | - |
ATP | 4000 | 1.5 | 13.64 | 30000 | 2200 |
CaM | 36 (4 sites) | 0.45 | 100 | 31 | 0.31 |
Fluo-4 | 50 | 0.75 | 307 | 276.7 | 0.9 |
Fluo-5F | 100 | 0.75 | 307 | 451.9 | 1.47 |
TnCI,hi | 140 (2 sites) | - | 100 | 7.1 | 0.071 |
TnCII,lo | 70 | - | 125 | 500 | 4 |
SLm | 86 | - | 125 | 1625 | 13 |
SRm | 47 | - | 115 | 100 | 0.87 |
SR | |||||
Cafree | 1000 | * | - | - | - |
CSQ | 120000 | - | 100 | 600000 | 600 |
Fluo-5N | 50 | * | 307 | 122956 | 400 |
For details, see Methods.