Table 2. Measurement of confocal point spread functions during live cell imaging.
FWHM (µm) | ||||
x | y | z | n | |
Water immersion | ||||
Coverslip | 0.38±0.03 | 0.35±0.02 | 1.7±0.2 | 6 |
Cell | 0.34±003 | 0.36±0.03 | 1.5±0.1 | 5 |
Oil immersion | ||||
Coverslip | 0.29±0.01 | 0.22±0.02 | 0.61±0.04 | 4 |
Cell | 0.32±0.01 | 0.32±0.02 | 1.19±0.06 | 5 |
Mean FWHM of PSFs on the coverslip and on top of live myocytes with oil and water immersion objectives (one S.E.M. shown). The focal plane is described by x and y, while the optical axis is described by z.