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| FWHM (µm) | ||||
| x | y | z | n | |
| Water immersion | ||||
| Coverslip | 0.38±0.03 | 0.35±0.02 | 1.7±0.2 | 6 |
| Cell | 0.34±003 | 0.36±0.03 | 1.5±0.1 | 5 |
| Oil immersion | ||||
| Coverslip | 0.29±0.01 | 0.22±0.02 | 0.61±0.04 | 4 |
| Cell | 0.32±0.01 | 0.32±0.02 | 1.19±0.06 | 5 |
Mean FWHM of PSFs on the coverslip and on top of live myocytes with oil and water immersion objectives (one S.E.M. shown). The focal plane is described by x and y, while the optical axis is described by z.
