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. 2013 Feb 28;8(2):e57430. doi: 10.1371/journal.pone.0057430

Figure 2. Transduction with lentiviral vector does not affect NPC differentiation in vitro.

Figure 2

NPCs were plated in the absence of EGF and FGF for 12 days. Immunocytochemistry for (A, D) GFAP, (B, E) ß3-tubulin, (C, F) c-myc of (A–C), control and (D–F), CD44-c-myc transduced actin eGFP cells (green). (G) Quantitative evaluation of the percentage of cells expressing the different markers over the total cell population identified by Hoechst staining (H+). Arrows in B and E point to cells enlarged in insets.