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. 2013 Feb 28;8(2):e57274. doi: 10.1371/journal.pone.0057274

Table 1. Effects of dual-labeling methods on tobacco pelleted seed germination.

Variety Treatment** Germination energy (%) Germination percentage (%) Germination index
MSYY85 Ck 95.1±4.2a∗ 98.9±1.1a 16.46±0.7a
T1 97.4±0.0a 99.8±0.2a 17.48±0.2a
T2 97.0±4.1a 98.3±2.9a 17.86±0.6a
T3 98.4±2.7a 98.4±2.7a 17.31±0.5a
T4 95.0±4.6a 98.6±2.4a 16.47±1.1a
HHDJY Ck 96.2±3.3a 98.1±3.3a 16.80±0.7a
T1 96.2±5.2a 98.4±2.2a 16.90±1.0a
T2 97.1±4.9a 97.1±4.9a 16.94±1.2a
T3 94.5±4.7a 98.6±1.3a 16.69±0.5a
T4 95.5±4.6a 97.8±3.9a 16.63±0.7a
*

Significant difference (α = 0.05, LSD) among treatments within the same variety.

**

Ck: 2 g seeds pelleted with 15 g bentonite and 84 g talc as a control; T1: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.6 g talc, 0.2 g Fluorescein and 4.2 g magnetic powder (FR and MP dual-labels); T2: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 77.08 g talc, 0.2 g Fluorescein and 6.72 g magnetic powder (FR and MP dual-labels); T3: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels); T4: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 77.28 g talc and 6.72 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels).