Table 1. Effects of dual-labeling methods on tobacco pelleted seed germination.
Variety | Treatment** | Germination energy (%) | Germination percentage (%) | Germination index |
MSYY85 | Ck | 95.1±4.2a∗ | 98.9±1.1a | 16.46±0.7a |
T1 | 97.4±0.0a | 99.8±0.2a | 17.48±0.2a | |
T2 | 97.0±4.1a | 98.3±2.9a | 17.86±0.6a | |
T3 | 98.4±2.7a | 98.4±2.7a | 17.31±0.5a | |
T4 | 95.0±4.6a | 98.6±2.4a | 16.47±1.1a | |
HHDJY | Ck | 96.2±3.3a | 98.1±3.3a | 16.80±0.7a |
T1 | 96.2±5.2a | 98.4±2.2a | 16.90±1.0a | |
T2 | 97.1±4.9a | 97.1±4.9a | 16.94±1.2a | |
T3 | 94.5±4.7a | 98.6±1.3a | 16.69±0.5a | |
T4 | 95.5±4.6a | 97.8±3.9a | 16.63±0.7a |
Significant difference (α = 0.05, LSD) among treatments within the same variety.
Ck: 2 g seeds pelleted with 15 g bentonite and 84 g talc as a control; T1: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.6 g talc, 0.2 g Fluorescein and 4.2 g magnetic powder (FR and MP dual-labels); T2: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 77.08 g talc, 0.2 g Fluorescein and 6.72 g magnetic powder (FR and MP dual-labels); T3: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels); T4: 2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 77.28 g talc and 6.72 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels).