Fig. 1.

Time-dependent increase in atrogin-1 and MuRF1 mRNA in response to alcohol. Male, fed rats were injected IP with 75 mmol/kg of ethanol, and gastrocnemius sampled at 1, 4, 8, or 24 h thereafter, and either atrogin-1 or MuRF1 quantitated by RNAse protection assay (RPA). Time-matched control animals were included for each time point. The subsequent analysis revealed no differences in control muscle between 0 and 8 h, and therefore, these control values were pooled and are presented as the “Ctr” value. Inset: representative RPA for atrogin-1, MuRF1, and the loading control L32 mRNA. Values are expressed as means ± SE; n = 6 or 7 rats per group, except for the Ctr group, in which the sample size was 12. *P < 0.05 compared with Ctr group.