Fig. 10.

Effect of acute alcohol intoxication on mRNA content of ubiquitin (Ub) and the content of Ub conjugates in gastrocnemius. Muscle was collected 8 h after in vivo intraperitoneal administration of alcohol (EtOH; 75 mmol/kg) or endotoxin (LPS; 1 mg/kg). Top: representative Northern blot for polyubiquitin mRNA in rats injected with saline, EtOH, and LPS, respectively. Middle: Ub mRNA content was quantitated, and the data were expressed in AUs determined by densitometry and normalized to 18S. Values are means ± SE; n = 5 or 6 per group. Bottom: representative Western blot for Ub conjugates in gastrocnemius muscle. Approximate molecular weights are indicated in left margin. P < 0.05 compared to time-matched control value.