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Effect of in vivo alcohol on protein balance in the incubated epitrochlearis muscle. Rats were administered alcohol in vivo (EtOH; 75 mmol/kg ip), and the epitrochlearis was isolated and incubated in vitro for the determination of protein synthesis (top) and protein degradation (middle). Values are expressed as means ± SE for n = 12 muscles per group. *P < 0.05 compared with time-matched control value. Bottom: representative RPA for atrogin-1, MuRF1, and the loading control L32 mRNA.
