Table 1.
Effect of gender, nutritional state, and alcohol metabolites on MuRF1 mRNA content in gastrocnemius in response to intraperitoneally administered alcohol
| Control | Alcohol | |
|---|---|---|
| Gender Study | ||
| Males | 1.00±0.12a | 3.89±0.35b |
| Females | 0.97±0.11a | 4.55±0.71b |
| Nutrition Study | ||
| Fasted | 1.00±0.07a | 5.21±0.63c |
| Fed | 0.73±0.05b | 3.87±0.53c |
| EtOH Metabolism Study | ||
| Control | 1.00±0.10a | 4.76±0.36b |
| 4-MP | 1.49±0.21a | 7.04±1.12b |
Values are expressed as means ± SE; n = 7 or 8 rats per treatment. Values represent arbitrary densitometric units from analysis of RNAse protection assay (RPA) for MuRF1 normalized for L32 (AU/L32). Similar alcohol (EtOH)-induced changes were detected for atrogin-1 (data not shown). 4-MP, 4-methylpyrazole.
a,b
Means with different letters are statistically different from one another within the same study (P < 0.05).