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Western blot reactivity of rabbit antisera (1/400 dilution) with ACP in lysates of meningococcal strains. Lanes L, protein markers; lane 1, rACP (0.1 µg); lane 2, MC58 ΔACP (20 µg); lanes 3 and 4, MC58 and MC168 (type 1; 20 µg); lanes 5 to 14, L2470, MC54, MC161, MC162, MC172, MC173, MC174, MC179, MC180, and MENC11 (type II; 20 µg); lane 15, MC90 (type III; 20 µg). Similar reactivities for all strains were observed with four additional and independent blots of the same samples.
