FIG. 3.

Flow cytometry and microarray analysis of distinct ATM populations. (A) Flow cytometric analysis of SVF cells obtained from aspirated AT showed that CD45⁺/CD206⁺ cell population expressed both CD14 and CD68, confirming that it is a population of monocytes/macrophages. The CD45⁺/CD206⁺/CD14⁺/CD68⁺ ATMs were further divided into 2 subpopulation, CD34⁺ population (11.1%) and CD34⁻ population (88.9%). (B) Correlation plot of the gene expression of 4 samples, CD34⁺/CD206⁺ ATMs, CD34⁻/CD206⁺ ATMs, ASCs, and PB-derived monocytes/macrophages (PB-MCs). CD34⁺/CD206⁺ ATMs, CD34⁻/CD206⁺ ATMs, and ASCs were freshly sorted from SVF and PB-MCs were obtained from PB. The plot revealed that the gene expression profiles of CD34⁺/CD206⁺ ATMs were more similar to that of ASCs compared with CD34⁻/CD206⁺ ATMs or PB-MCs. All array data sets were deposited into the National Center for Biotechnology Information Gene Expression Omnibus database (ID no. GSE30742, www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE30742). (C) Cluster analysis of the 4 samples. This analysis confirmed that CD34⁺/CD206⁺ ATMs show similar gene expression pattern as ASCs rather than CD34⁻/CD206⁺ ATMs. (D) Gene expression of mesenchymal, pericyte, endothelial, and monocyte/macrophage markers in samples. Both CD34⁺/CD206⁺ ATMs and ASCs highly expressed mesenchymal markers and pericytes markers. Only CD34⁺/CD206⁺ ATMs showed endothelial markers. Color images available online at www.liebertpub.com/scd