FIG. 5.

Whole-mount histological examination for localization of ATMs (A) Immunostaining of whole-mount AT indicated that CD206⁺ ATMs (CD14⁺/CD206⁺ cells) located mostly periendothelially in capillaries and a small number of CD206⁺ATMs were seen also in interstitial spaces between adipocytes. Lectin (blue) was used to visualize vascular endothelium. Scale bar=50 μm. (B) CD206⁺ ATMs (CD206⁺/CD45⁺ cells; arrowhead) localized outside of endothelial cells, while intravascular hematopoietic cells (CD45⁺ circulating leukocytes) did not express CD206. Scale bar=25 μm. (C) ATMs (CD206⁺ cells), coexpressing CD34, were found to be localized perivascularly, although not all ATMs expressed CD34. Scale bar=25 μm. (D) Staining images with isotype control. Scale bar=50 μm. Color images available online at www.liebertpub.com/scd