FIG. 2.
DAZL enhances PLC formation. SSCs were transduced with DAZL- or GFP (Con)-lentivirus and induced to differentiate. PLCs started to appear in the cultures from ∼D14 of differentiation onward. (A) Representative images of large, medium, and small PLC clusters. (B) Percentages of differentiating cell dishes showing large-, medium-, and small-PLC clusters in DAZL- and control (GFP)-transduced groups, respectively, at D24 of differentiation. (C) PLCs were harvested and counted, and cell numbers are shown. The total number of cells, including PLCs and supporting cells, is 12×105 cells/mL. Data represent the mean±SEM of 3 independent experiments, and an asterisk (*) indicates a significant difference from the control (B, C: t-test comparing the DAZL and control groups; P<0.05). Scale bar=100 μm. SSC, skin-derived stem cells.