FIG. 3.

DAZL enhances early germ cell marker expression. At D24 of differentiation, PLCs were collected, and RT-PCR was performed. (A) Relative mRNA levels of germ cell markers in DAZL- and GFP (Con)-transduced groups. (B) Representative images of Fragilis- and/or GFP-positive cells. (C) Fragilis-positive cell ratio in the DAZL- and GFP (con)-transduced groups. Data represent the mean±SEM of 4 independent experiments, and an asterisk (*) indicates a significant difference from the control (t-test comparing the DAZL groups and their respective controls; P<0.05). Scale bar=20 μm. PCR, polymerase chain reaction; RT, reverse transcription. Color images available online at www.liebertpub.com/scd