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. 2012 Dec 15;125(24):6094–6104. doi: 10.1242/jcs.113019

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© 2012. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 6. — Characterization of macroH2A-depleted iPSCs. (A) qRT-PCR analysis of retrovirus expression in NSCs, NS Pre-iPSCs and in iPSCs derived from macroH2A.1 or macroH2A.2 NSCs. Klf4 retrovirus (RetrKlf4) is in blue and Oct4 retrovirus (RetrOct4) in red. (B) Gene expression analysis of the iPSCs depleted of macroH2A. qRT-PCR analysis of pluripotency gene expression in NSCs, ESCs and iPSCs derived from macroH2A.1 or macroH2A.2 NSCs. Endogenous Oct4 (EndOct4) is in blue, Rex1 in red, Klf4 in purple and Nanog in green. (C) Tissues of the three embryonic germ layers are present in the teratomas derived from macroH2A.2 shRNA expressing iPSCs. (D) Contribution of macroH2A-depleted iPSCs to chimeric mice. These were obtained after blastocyst injection of macroH2A shRNA iPSCs into C57/BL6 blastocysts. Agouti coat color indicates chimerism (arrows). Error bars indicate s.d.