Fig. 2.

HPat is expressed in the larval nervous system. (A) Whole-mount RNA in situ hybridization of w¹¹¹⁸ larval brains stained with either antisense or sense riboprobes directed against the hpat mRNA. (B) Larval ventral ganglion stained with rat antibodies against HPat (green) and/or FMRP (red) showing punctate cytoplasmic staining in the soma of most neurons. There is a significant amount of overlap between HPat and FMRP in the merged image. Scale bar: 20 µm. (C) NMJs double stained with antibodies against HPat (green) and postsynaptic Dlg (red). HPat exhibits punctate perinuclear localization in muscle but is not enriched at the NMJ. Scale bar: 20 µm. (D) Peripheral nerves exiting the ventral ganglion showing localization of both HPat (green) and FMRP (red). In the merged images in B and D, yellow spots indicate co-localization between HPat- and FMRP-containing punctae. Scale bar: 10 µm.