Table 1.
GST-Src SH2 | GST-Src SH2 + C112 Peptide (HCSQV) | GST-Src SH2 + C246 Peptide (CALG) | |||||||||||||||
Ion | Exp. Da | Obs. Da | Ion | Exp. Da | Obs. Da | Ion | Exp. da | Obs. Da | Ion | Exp. Da | Obs. Da | Ion | Exp. Da | Obs. Da | Ion | Exp. Da | Obs. Da |
b1 | 57.02 | ---- | y1 | 146.11 | 147.11 | b1 | 57.02 | ---- | y1 | 146.11 | ---- | b1 | 57.02 | ---- | y1 | 146.11 | 147.11 |
b2 | 128.06 | 129.07 | y2 | 217.14 | 218.15 | b2 | 128.06 | 129.07 | y2 | 217.14 | 218.15 | b2 | 128.06 | 129.07 | y2 | 217.14 | 218.15 |
b3 | 291.12 | 292.13 | y3 | 331.19 | 332.19 | b3 | 291.12 | 292.13 | y3 | 331.19 | 332.19 | b3 | 291.12 | 292.13 | y3 | 331.19 | 332.19 |
b4 | 394.13 | 395.14 | y4 | 446.21 | 447.22 | b4 | 965.36 | 965.36* | y4 | 446.21 | 447.22 | b4 | 755.29 | 755.28* | y4 | 446.21 | 447.21 |
b5 | 507.22 | 508.22 | y5 | 593.28 | 594.29 | b5 | 1078.45 | 1078.44* | y5 | 593.28 | 594.29 | b5 | 868.37 | 868.36* | y5 | 593.28 | 594.28 |
b6 | 594.25 | ---- | y6 | 708.31 | 709.32 | b6 | 1165.47 | 1165.48* | y6 | 708.31 | 709.32 | b6 | 955.40 | 955.38* | y6 | 708.31 | 709.31 |
b7 | 693.32 | 694.32 | y7 | 795.34 | 796.35 | b7 | 1264.55 | 1264.55* | y7 | 795.34 | 796.35 | b7 | 1054.47 | 1054.46* | y7 | 795.34 | 796.34 |
b8 | 780.35 | 781.36 | y8 | 894.41 | 895.42 | b8 | 1351.58 | 1351.58* | y8 | 894.41 | 895.42 | b8 | 1141.50 | 1141.50* | y8 | 894.41 | 895.40 |
b9 | 895.38 | ---- | y9 | 981.44 | 982.45 | b9 | 1466.61 | 1466.60* | y9 | 981.44 | 982.45 | b9 | 1256.53 | 1256.51* | y9 | 981.44 | 982.44 |
b10 | 1042.44 | 1043.45 | y10 | 1094.52 | 1095.53 | b10 | 1613.67 | 1613.67* | y10 | 1094.52 | 1095.53 | b10 | 1403.60 | 1403.58* | y10 | 1094.52 | 1095.52 |
b11 | 1157.47 | 1158.48 | y11 | 1197.53 | 1198.54 | b11 | 1728.70 | 1728.70* | y11 | 1768.76 | 1768.76* | b11 | 1518.62 | 1518.63* | y11 | 1558.69 | 1558.68* |
b12 | 1271.51 | 1272.52 | y12 | 1360.60 | 1361.60 | b12 | 1842.74 | ---- | y12 | 1931.83 | 1931.83* | b12 | 1632.67 | 1632.66* | y12 | 1721.75 | ---- |
b13 | 1342.55 | 1343.56 | y13 | 1431.63 | 1432.64 | b13 | 1913.78 | 1913.78* | y13 | 2002.86 | ---- | b13 | 1703.71 | 1703.70* | y13 | 1792.79 | ---- |
b14 | 1470.65 | ---- | y14 | 1488.66 | ---- | b14 | 2041.88 | ---- | y14 | 2059.89 | ---- | b14 | 1831.80 | ---- | y14 | 1849.81 | ---- |
Non-bold numerals indicate expected ion masses; bold numerals indicate observed ion masses; the asterisks indicate the peptide modified masses.