Figure 1.

Amino acid-substituted CLV3 constructs and transgenic analyses in the wild type. A, Constructs made for two-dimensional substitutions of CLV3. A full-length CLV3 genomic sequence (CLV3F) including endogenous regulatory elements was used as the template for first dimensional Ala substitutions in the 12-amino acid peptide-coding region of CLV3 (named CLV3_Ala1–12) and second dimensional substitutions of the Gly residue at position 6 with all other 18 possible proteinaceous amino acids. B, Effects of 12 Ala-substituted CLV3s in generating dominant-negative clv3-like multicarpel phenotypes in the wild type. For each Ala-substituted CLV3, at least 30 transformants were obtained. The wild-type (Ler), clv3-2, and CLV3F transgenic plants were used as controls. C and D, Wild-type plant (C) with two-carpel siliques (inset in C) and the CLV3_Ala6 transgenic plant (D) with multicarpel siliques (inset in D). Bars = 10 mm for C and D and 0.7 mm for the insets. E and F, Inflorescences of the wild type (E) and the BC1 plant of the CLV3_Ala6 transgenic line (F). Bars = 2 mm.