Figure 3.

Effects of CLV3_Thr6 in generating the clv3-like phenotype. A, Among 43 individual plants transformed with CLV3_Thr6 examined, 30 showed a multicarpel clv3-like phenotype. Note that wild-type Arabidopsis has two carpels in siliques, and the antagonistic effect was observed in transgenic lines with more than two carpels. The diamond indicates the average carpel number, while the top and bottom bars represent the most and least carpel numbers, respectively. B, Transverse sections through siliques from CLV3_Thr6 transgenic plants with three (middle) and seven (right) carpels compared with the wild type (left) with two carpels. The red curves indicate the carpels. Bars = 200 µm. C, Inflorescences of wild-type, CLV3_Thr6 transgenic, and clv3-2 plants. Compared with the wild type (left), inflorescences in CLV3_Thr6 transgenic plants (middle) had supernumerary flowers, as observed in clv3-2 mutants (right). Bars = 3 mm. D, A DIC image showing the enlarged SAM in the CLV3_Thr6 transgenic plant (middle) compared with those from the wild type (left) and clv3-2 (right). Arrowheads indicate the margins of SAMs. Bars = 50 µm.