Figure 5.
Preparation of β-1,3-galactooligosaccharides (β-1,3-Galn). A, β-1,3-Galn prepared from β-galactan IV by partial acid hydrolysis were separated by gel-permeation chromatography on a Bio-Gel P-2 column. B to D, β-1,3-Gal7 (B), β-1,3-Gal8 (C), and β-1,3-Gal9 (D) were further purified on the same column. In the Yariv assay, the fractions indicated by arrowheads were used. E, To confirm purity, purified β-1,3-Galn were coupled at their reducing terminals with p-aminobenzoic acid ethyl ester and analyzed on the HPLC system. V0 and Vi indicate void volume and inner volume, respectively.