Figure 1.
Expression patterns of AtROP6 in the shoot and the root. A, B, D, E, G, and H, pROP6>>GUS. C, F, J, K, and L, pROP6>>GFP-rop6CA. I, pROP6>>DsRed. A to C and F, In rosette leaves, ROP6 expression is primarily restricted to hydathodes, whereas in cauline leaves, it is detected throughout the epidermis. D, ROP6 expression could not be detected in the inflorescence meristem and young flowers. E, In mature flowers, expression is detected in stamen filaments and pollen grains, stigma, style, and weakly in ovules. G, In the root, expression is detected in the root cap, in and around the quiescent center; it is absent from the cell division and transition zone, and it reappears in the stele in the differentiation zone. H and I, Expression then drops again and reappears in LRI and founder cells. The arrow denotes a protoxylem file. J, Expression of ROP6 is induced by auxin and can be detected in pericycle cells as early as 3 h after induction. K, After 6 h, expression is spread to more cells in the pericycle. L, Twenty-four hours after induction, expression is spread throughout the pericycle and extensive cell divisions are detected. Bars = 100 μm (A), 50 μm (B, D, E, and G–L), and 20 μm (C and F). For more information, see Supplemental Figure S2.