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. 2013 Jan 24;161(3):1278–1290. doi: 10.1104/pp.112.203760

Figure 6.

Figure 6.

Analysis of GhFLA1 expression and function in overexpression and RNAi transgenic cotton plants. A, Maps of the GhFLA1 overexpression construct (top) and the RNAi construct (bottom). LB, Left border; ORF, open reading frame; RB, right border. B, Real-time RT-PCR analysis of the expression of GhFLA1 in overexpression plants (T2 generation). RNA was isolated from 10-DPA fibers. C, Real-time RT-PCR analysis of the expression of GhFLA1 in RNAi plants (T2 generation). RNA was isolated from 10-DPA fibers. D, Measurement of fiber length at 1 and 2 DPA (n ≥ 50 cotton ovules per line). E, Paraffin sections of 1- and 2-DPA cotton ovules. F, Measurement of fiber length at maturation (n ≥ 50 cotton ovules per line). G, Mature fibers of GhFLA1 RNAi plants. H, Mature fibers of GhFLA1 overexpression plants. Bars = 75 μm in E and 1 cm in G and H. Values shown are means ± sd. Independent Student’s t tests demonstrated that there was a significant difference (*P < 0.05) or a very significant difference (**P < 0.01) between transgenic fibers and the wild type (WT). OL, GhFLA1 overexpression transgenic lines; RL, GhFLA1 RNAi transgenic lines.